Composite

Part:BBa_K824008:Experience

Designed by: Steven Allen   Group: iGEM12_Gaston_Day_School   (2012-10-01)

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Please enter how you used this part and how it worked out.

Applications of BBa_K824008

User Reviews

UNIQ78c7417190188cc3-partinfo-00000000-QINU UNIQ78c7417190188cc3-partinfo-00000001-QINU Oct. 2014: Part K1320000 contains the same cadmium responsive promoter and GFP but includes sensitivity tuners. It is far more sensitive than this part (2.5mM Cd).


Oct. 2012: We grew liquid cultures for 24 hours with varying concentrations of Cadmium Nitrate in the presence of our cells. After growth, the tests were spun down, then resuspended in 1X PBS (Phosphate Buffer Solution). The results were measured in fluorescence per OD 600. The graph below shows our results.


Cadgraphuse.jpg